|
KMID : 0895820130230010001
|
|
Journal of Oriental Rehabilitation Medicine
2013 Volume.23 No. 1 p.1 ~ p.13
|
|
|
Effect of Tetramethylpyrazine on Neuronal Apoptosis in Spinal Cord Compression Injury of Rats
|
|
Jo Jong-Jin
Kim Seung-Hwan
Lee Joon-Seok
Shin Jung-Won
Kim Seong-Joon
Sohn Nak-Won
|
|
|
Abstract
|
|
|
Objectives：The pathophysiology of acute spinal cord injury(SCI) may be divided into primary and secondary mechanisms of injury．The secondary mechanism involves free radical formation, excitotoxicity, inflammation and apoptotic cell death, and sets in minutes after injury and lasts for weeks or months．During this phase the spinal tissue damages are aggravated．Therefore, secondary mechanisms of injury serve as a target for the development of neuroprotective drug against SCI．The present study investigated the effect of tetramethylpyrazine(TMP), an active ingredient purified from the rhizome of Ligusticum wallichii(川芎, chuanxiong), on neuronal apoptosis in spinal cord compression injury in rats．
Methods：SCI was subjected to rats by a static compression method(35 g weight, 5 mins) and TMP was treated 3 times(30 mg/kg, i.p.) during 48 hours after the SCI．
Results：TMP ameliorated the tissue damage in peri-lesion of SCI and reduced TUNEL-labeled cells both in gray matter and in white matter significantly．TMP also attenuated Bax-expressed motor neurons in the ventral horn and preserved Bcl-2-expressed motor neurons．
Conclusions：These results indicate that TMP plays a protective role in apoptotic cell death of neurons and oligodendrocytes in spinal cord injury．Moreover, it is suggested that TMP and TMP-containing chuanxiong may potentially delay or protect the secondary spinal injury．
|
|
|
KEYWORD
|
|
|
Tetramethylpyrazine, Ligusticum wallichii, Spinal cord injury, TUNEL, Bax, Bcl-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|